The authors wish to acknowledge The Childrens Memorial Health Institute, Warsaw, Poland for the access to Q-TOF LC/MS; mass spectrometer purchase was supported by European POIG

The authors wish to acknowledge The Childrens Memorial Health Institute, Warsaw, Poland for the access to Q-TOF LC/MS; mass spectrometer purchase was supported by European POIG

May 30, 2023
Notably, we found that the presence of sodium cholate in the assay buffer is definitely imperative to achieve separation and prevent aggregation of lipids

Notably, we found that the presence of sodium cholate in the assay buffer is definitely imperative to achieve separation and prevent aggregation of lipids

May 29, 2023
The reaction was stirred at 23 C for 30 min

The reaction was stirred at 23 C for 30 min

May 28, 2023
The total email address details are representative of three separate experiments

The total email address details are representative of three separate experiments

May 26, 2023
A) PCR was performed using primers for the wild-type Crry gene as well as for the Neo put utilized to disrupt the gene

A) PCR was performed using primers for the wild-type Crry gene as well as for the Neo put utilized to disrupt the gene

May 25, 2023
Remember that even after 3 times of demineralization (arrow), abundant Ca2+ remains in the bone tissue core even now

Remember that even after 3 times of demineralization (arrow), abundant Ca2+ remains in the bone tissue core even now

May 24, 2023

The next primers for GOLPH3 and POMGnT1 were designed: GOLPH3 primer, 5-CAGCCACGTAATCCAGATGAT-3 (forwards) and 5-ACCCTGATGGAGGAAGTGCT-3 (reverse); POMGnT1 primer, 5-CCTGGAACCGTGTTGAACTT-3 (forwards) and 5-CGATCCTACCACTTTGGCAT-3 (invert)

The next primers for GOLPH3 and POMGnT1 were designed: GOLPH3 primer, 5-CAGCCACGTAATCCAGATGAT-3 (forwards) and 5-ACCCTGATGGAGGAAGTGCT-3 (reverse); POMGnT1 primer, 5-CCTGGAACCGTGTTGAACTT-3 (forwards) and 5-CGATCCTACCACTTTGGCAT-3 (invert). T7 primers to...