A single additional base found in the leader sequence of the primary reference sequence in the IMGT database was not replicated in a number of other germline gene entries suggesting that it may symbolize a sequencing error or an uncommon allelic variant

A single additional base found in the leader sequence of the primary reference sequence in the IMGT database was not replicated in a number of other germline gene entries suggesting that it may symbolize a sequencing error or an uncommon allelic variant. variant (SNP rs12588974) at base 299, indicative of the IGHV1-2*01 or IGHV1-2*05 alleles is present at about 5% in European populations. Bases 233 and 234 (SNPs rs782139757 and rs1425538657), that individual these two alleles remains as T and G, respectively, at very high frequency in most populations suggesting that IGHV1-2*01 is not common in these populations (not shown). All sequence variants of the illustrations of SNPs are indicated as seen in the reversed strand, hence they are complementary to the base of the coding strand. Image_3.pdf (334K) GUID:?5EF150A8-19FA-413D-B723-6D503BE2F701 Supplementary Physique 4: Allelic variants of IGHV1-3 as defined by IMGT are illustrated. Variability of some of the positions of these genes in samples obtained in different geographical locations as illustrated by the ENSEMBL browser (release 101, August 2020) (21) is usually shown. Only bases 6, 12, 167, 208, 291 and 296 [IMGT numbering nomenclature (20)] of this gene display frequencies of variance >1% in the 1000 Genomes Project. Variants indicative of the IGHV1-3*02 allele are present at about 40%. All sequence variants of the illustrations of SNPs are indicated as seen in the reversed strand, hence they are complementary to the base of the coding strand. Image_4.pdf ASP 2151 (Amenamevir) (557K) GUID:?4BA152B1-63CA-417C-88EA-8CBF6E5FACB1 Supplementary Physique 5: Allelic variants of IGHV4-4 as defined by IMGT are illustrated. Variability of some of the positions of these genes in samples obtained in different geographical locations as illustrated by the ENSEMBL browser (release 101, August 2020) (21) is usually shown. Analysis of this gene is ASP 2151 (Amenamevir) usually complicated by considerable similarity with alleles of IGHV4-59 and IGHV4-61, alleles of which are also shown. A few of the positions of IGHV4-4 that display frequencies of variance >1% in all ASP 2151 (Amenamevir) populations in the 1000 Genomes Project are shown. Note that variants at bases 46 and 308 [IMGT numbering nomenclature (20)], indicative of the IGHV4-4*01 allele are present at about 3-4% in European populations. All sequence variants of the illustrations of SNPs are indicated as seen in the reversed strand, hence they are complementary INSR to the base of the coding strand. Image_5.pdf (555K) GUID:?5DCC7D88-155E-45A4-AF44-C3BD2B443E7F Supplementary Physique 6: Allelic variants of ASP 2151 (Amenamevir) IGHV7-4-1 as defined by IMGT are illustrated. Variability of some of the positions of these genes in samples obtained in different geographical locations as illustrated by the ENSEMBL browser (release 101, August 2020) (21) is usually shown. Sequence variance in base 274 [IMGT numbering nomenclature (20)] suggests that the base associated to IGHV7-4-1*01 is usually more common than the base associated to other alleles of this gene in most populations. All sequence variants of the illustrations of SNPs are indicated as seen in the reversed strand, hence they are complementary to the base of the coding strand. Image_6.pdf (278K) GUID:?9C5949AC-E2FD-4888-97BC-DD770BF18464 Supplementary Figure 7: High resolution structures of five antibodies with a heavy chain variable domain name encoded by a gene derived from IGHV7-4-1. Heavy chain CDR3 is shown at the top of each structure in red. The side chain of residue 92 (in all cases a serine), located far from the antibody binding site is usually shown in green (carbon) and reddish (oxygen). Structures include PDB entries 4D9Q (A), 4EOW (B), 5CGY (C), 5ZMJ (D), and 6B5R (E). Image_7.pdf (2.8M) GUID:?F6A16093-342A-41F8-98E5-EF0021DFA718 Supplementary Figure 8: Translated sequences of productive IgA and IgG-encoding reads derived from NGS data sets of two subjects (donors 2 and 4) that both have IGHV7-4-1*01 but not IGHV7-4-1*02 in their genotype (15). The sequencing protocol (19) allowed for determination of the sequence from the end of framework 1 and extended into the first constant domain of the heavy chain. The sequences encoded by IGHV7-4-1*01 and IGHV7-4-1*02 are shown on top of the physique. Residue 92 is usually highlighted by an arrow. Image_8.pdf (4.6M) GUID:?A8A3C482-01A4-449C-822B-111F10D7FA05 Supplementary Figure 9: Linkage equilibrium involving SNPs associated to IGHV1-2*05 and IGHV4-4*01 is identified in many populations [ENSEMBL browser (release 101, August 2020) (21)]. SNP rs12588974 (base 299 of IGHV1-2) separates IGHV1-2*05 from other commonly.