In the PTS reaction, the N-terminal and the C-terminal a part of a split intein (intein-N and intein-C) are fused to the target proteins and ligated with each other to form a peptide bond, and the intein moiety is released without any structural trace at the ligation site
In the PTS reaction, the N-terminal and the C-terminal a part of a split intein (intein-N and intein-C) are fused to the target proteins and ligated...